Qrf Antibody Test Uncut Sheet

The detection of sheep pox virus antibody is composed of a microplate pre-coated with sheep pox nuclear protein antigen, enzyme markers and other supporting reagents, and the principle of enzyme-linked immunoassay (ELISA) is used to detect the sheep pox antibody in the sheep serum sample. During the experiment, the control serum and the sample to be examined are added to the microplate plate, and if the sample contains sheep pox antibody after incubation, it will be bound to the antigen on the microplate plate, and other components that are not bound will be removed after washing; Then add the enzyme marker to specifically bind to the antigen-antibody complex on the microplate plate; The unbound enzyme markers were then removed by washing, and TMB substrate solution was added to the wells, and the blue product was formed by the reaction of the microplate conjugates, and the color depth was positively correlated with the specific amount of antibodies contained in the sample. After the termination solution was added to terminate the reaction, the product turned yellow; The absorbance value in each reaction well is determined by a microplate reader at a wavelength of 450 nm to determine whether the sample contains sheep pox antibodies.