Detailed description
(1) For sample collection and screening test, only a single blood sample needs to be collected. However, if it is necessary to judge the immune status of people infected with the virus, it is necessary to take samples from suspected rubella patients within 3 days after the onset of the rash and the following 14 to 21 days for simultaneous detection.
(2) The same as the general ELISA, add PBS 50 in each hole of the control and sample μ l. Continue adding sample 10 μ l. Heat at 25 ℃ for 45 min, wash and dry.
(3) Add enzyme markers to each well 250 μ l. The same method is used for heat preservation and washing.
(4) Add pNPP substrate solution 250 μ l. After heat preservation and washing by the same method, add 1mol/L sodium hydroxide 50 μ L Stop the reaction, measure the absorbance value of each hole at 405nm, and judge the result of the tested sample.
(5) If it is a positive result, the sample can be further diluted to determine the antibody titer, compare the results of two consecutive samples, and judge
Customized Contents
Uncut Sheet Rapid Test Manufacturing Process
